For each immobilized enzyme optimization of activity and stability is performed. After immobilization all Immozymes are washed extensively. Finally, they are dried yielding an enzyme that can be stored for prolonged times and used in water-free media.
enzyme carriers
ChiralVision’s immobilized proteases are ideal for resolutions under aqueous, biphasic and water-free conditions for hydrolysis, esterification and amidation of a wide variety of substrates. These immozymes are available individually in any quantity or in a screening kit:
-22 immobilized proteases / kit
-1 gram each
-covalently bound on IB-COV-2
-particle size 150-500 μm
-instructions
HYDROLYSIS
ELU activity: hydrolysis of (-)L-ethyllactate (“EL” Units) with water to lactic acid.
1 ELU unit = 1 μmol lactic acid released per minute / g immobilized enzyme.
Conditions: 30% ethyllactate in buffer at 25°C and pH 6.8. Reaction time: 5 minutes
ESTERIFICATION
BYU activity: esterification of BOC-tyrosine (“BY” Units) with 1,3-propandiol to BOC-Tyr-OC3H6OH.
1 BYU unit = 1 μmol BOC-tyrosine ester produced per minute / g immobilized enzyme.
Conditions: 10% Boc-tyrosine in 1,3-propandiol (W/V) with 2.5% water at 60°C. Reaction time: 1 hour.
AMIDATION
GAU activity: amidation of BOC-Tyr-OC3H6OH with glycinamide to the corresponding dipeptide.
1 GAU unit = 1 μmol BOC-tyrosine amide produced per minute / g immobilized enzyme.
Conditions: 10% Boc-tyrosine ester and glycinamide (2M) in 1,3-propandiol (W/V) with 2.5% water at 40°C.
Reaction time: 1-24 hour to reach maximum conversion.
Indicative activities of immobilized proteases in:
hydrolysis (ELU), esterification (BYU) and amidation (GAU).
Immozymes Protease product price list (PDF)