Discover immobilized proteases
ChiralVision’s immobilized proteases are ideal for resolutions under aqueous, biphasic and water-free conditions for hydrolysis, esterification and amidation of a wide variety of substrates. These immozymes are available individually in any quantity or in a screening kit:
-22 immobilized proteases / kit
-1 gram each
-covalently bound on IB-COV-2
-particle size 150-500 μm
ELU activity: hydrolysis of (-)L-ethyllactate (“EL” Units) with water to lactic acid.
1 ELU unit = 1 μmol lactic acid released per minute / g immobilized enzyme.
Conditions: 30% ethyllactate in buffer at 25°C and pH 6.8. Reaction time: 5 minutes
BYU activity: esterification of BOC-tyrosine (“BY” Units) with 1,3-propandiol to BOC-Tyr-OC3H6OH.
1 BYU unit = 1 μmol BOC-tyrosine ester produced per minute / g immobilized enzyme.
Conditions: 10% Boc-tyrosine in 1,3-propandiol (W/V) with 2.5% water at 60°C. Reaction time: 1 hour.
GAU activity: amidation of BOC-Tyr-OC3H6OH with glycinamide to the corresponding dipeptide.
1 GAU unit = 1 μmol BOC-tyrosine amide produced per minute / g immobilized enzyme.
Conditions: 10% Boc-tyrosine ester and glycinamide (2M) in 1,3-propandiol (W/V) with 2.5% water at 40°C.
Reaction time: 1-24 hour to reach maximum conversion.
Indicative activities of immobilized proteases in:
hydrolysis (ELU), esterification (BYU) and amidation (GAU).
Immozymes Protease product price list (PDF)