Immozymes
Immobilized lipases, proteases and other enzymes
Immobeads
Immobilization and purification of enzymes and proteins in general requires special carriers. ChiralVision offers carriers with high porosity and various binding modes for optimal immobilization and purification.
Our carriers with binding through epoxides, adsorption, cationic groups, anionic groups and His-tags cover the most important range of binding options.
Chirals
chiral compounds
Immobeads
ChiralVision has an extensive range of enzyme carriers with various surface functionalizations. These are based on acrylic polymers but also on silica and cellulose. In collaboration with Sunresin we also offer their line of life science resins making the range of options for your application very complete.
All carriers are available separately and in convenient screening kits (see bottom page).
Immobeads for covalent, adsorption and ionic immobilization
Macroporous carriers for enzyme immobilization or protein purification. Available in various screening kit combination or separate in bulk.
| Covalent | |
| All Immobeads are available in bulk | |
| Product# (Sunresin#) | Bead description |
| IB-COV-1 | Covalent, very hydrophobic, polyacrylic, low epoxide and high butyl |
| IB-COV-2 | Covalent, hydrophilic, polyacrylic, epoxide and low butyl |
| IB-COV-3 | discontinued, replaced by IB-COV-8 |
| IB-COV-4 | Covalent, very hydrophobic, polyacrylic, low epoxide and octadecyl |
| IB-COV-5 | Covalent, hydrophobic, polyacrylic, epoxide and hexadecyl/octadecyl |
| IB-COV-6 | Covalent, very hydrophilic, polyacrylic, epoxide and hydroxyethyl |
| IB-COV-7 (EMC7014M) | Covalent, hydrophilic, polyacrylic, epoxide |
| IB-COV-8 | Covalent, very hydrophilic, polyacrylic, epoxide |
| IB-COV-9 | Covalent, extremely hydrophilic, silica, propyl epoxide |
| IB-COV-10 | Covalent, extremely hydrophilic, cellulose, epoxide, anionic |
| IB-COV-11 (EMC7025) | Covalent, hydrophilic, polyacrylic, epoxide |
| IB-COV-12 (EMC7032) | Covalent, hydrophobic, polyacrylic, epoxide, butyl |
| Adsorption | |
| All Immobeads are available in bulk | |
| Product# (Sunresin#) | Bead description |
| IB-ADS-1 | Adsorption, hydrophobic, polyacrylic, hexadecyl/octadecyl |
| IB-ADS-4 | discontinued, replaced by IB-ADS-10 |
| IB-ADS-8 | Adsorption, hydrophobic, polyacrylic, low butyl |
| IB-ADS-9 | Adsorption, extremely hydrophobic, polyacrylic, high butyl |
| IB-ADS-10 (EMC1040) | Adsorption, hydrophilic, polystyrene, styrene, methyl |
| IB-ADS-11 (EMC7528) | Adsorption, hydrophobic, polyacrylic, octadecyl |
| IB-ADS-12 (EMC1020) | Adsorption, hydrophobic, polystyrene / DVB, styrene |
| Cationic | |
| All Immobeads are available in bulk | |
| Product nr. | Bead description |
| IB-CAT-1 | Cationic, strong, polystyrene, sulphonic |
| Anionic | |
| All Immobeads are available in bulk | |
| Product# (Sunresin#) | Bead description |
| IB-ANI-5 (EMC7120M) | Anionic, hydrophobic, polyacrylic, primary, secondary amine |
| IB-ANI-6 (EMC7424) | Anionic, hydrophilic, polystyrene / DVB, tertiary amine |
| IB-ANI-7 | Anionic, very hydrophilic, polyacrylic, primary, secondary amine |
| IB-ANI-8 | Anionic, very hydrophilic, polyacrylic, primary amine |
| IB-ANI-9 | Anionic, hydrophobic, polyacrylic, secondary, tertiary amine |
| IB-ANI-10 | Anionic, hydrophilic, polyacrylic, primary, secondary amine |
| IB-ANI-11 | Anionic, hydrophobic, polyacrylic, primary, secondary amine |
| IB-ANI-12 | Anionic, extremely hydrophilic, silica, amino propyl |
| IB-ANI-13 | Anionic, extremely hydrophilic, cellulose, primary, secondary amine |
| IB-ANI-14 (EMC7435) | Anionic, hydrophobic, polystyrene / DVB, quaternary amine |
| IB-ANI-15 (EMC7225M) | Anionic, hydrophilic, polyacrylic, short diamine spacer |
Purolite has discontinued various lifescience products. ChiralVision offers the following drop-in replacement beads:
| ChiralVision product # | Replaces Purolite product # |
| IB-COV-8 | ECR8215 |
| IB-ADS-10 | ECR1030 |
| IB-ADS-11 | ECR8806 |
| IB-ADS-12 | AP1090 |
| IB-HIS-8 | Chromalite MIDA Ni |
Contact us for remaining Purolite stock!
Immobeads for His-tag affinity purification and immobilization
The Immobead His-tag product line is suited for binding enzymes and proteins with a His-tag domain. The carriers have nickel attached via an iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA) group and are ready for use. If you prefer to obtain these carriers without a metal ion or with a different metal please contact us. The products are available as screening kit or individual in various quantities as well as in bulk.
| Product# (Sunresin#) | Bead description |
| IB-HIS-1 | HIS-tag, very hydrophobic, polyacrylic, low IDA-nickel, high butyl |
| IB-HIS-2 | HIS-tag, hydrophilic, polyacrylic, IDA-nickel, low butyl |
| IB-HIS-3 | HIS-tag, very hydrophobic, polyacrylic, low IDA-nickel, octadecyl |
| IB-HIS-4 | HIS-tag, hydrophobic, polyacrylic, IDA-nickel, hexadecyl/octadecyl |
| IB-HIS-7 | HIS-tag, very hydrophilic, polyacrylic, IDA-nickel, hydroxyethyl |
| IB-HIS-8 | HIS-tag, very hydrophilic, polyacrylic, IDA-nickel |
| IB-HIS-9 | HIS-tag, very hydrophilic, polyacrylic, IDA-nickel, ammonia quenched |
| IB-HIS-11 | HIS-tag, hydrophilic, anionic, polyacrylic, diamine, NTA-nickel |
| IB-HIS-14 | HIS-tag, hydrophilic, anionic, polyacrylic, NTA-nickel |
| IB-HIS-15 | HIS-tag, extremely hydrophilic, anionic, silica, aminopropyl, NTA-nickel |
| IB-HIS-16 | HIS-tag, extremely hydrophilic, anionic, cellulose, NTA-nickel |
| IB-HIS-17 (Chelex7350) | HIS-tag, hydrophilic, anionic, polyacrylic, IDA and Nickel |
| IB-HIS-18 | HIS-tag, hydrophobic, anionic, polyacrylic, diamine, NTA-nickel |
| IB-HIS-19 | HIS-tag, very hydrophilic, anionic, polyacrylic, diamine, NTA-nickel |
| IB-HIS-20 | HIS-tag, very hydrophilic, polyacrylic, ammonia, NTA-nickel |
| IB-HIS-21 | HIS-tag, extremely hydrophilic, silica, propyl, IDA-nickel |
| IB-HIS-22 | HIS-tag, extremely hydrophilic, cellulose, IDA-nickel |
| IB-HIS-24 | HIS-tag, very hydrophilic, polyacrylic, diamine, EDTA-nickel |
His-tag Immobead product list (PDF)
Immobeads: screening kits
Various screening kits are available with a variation in binding types:
| Catalog No. | Immobead enzyme carrier Kit - Pricing in euro (ex. shipping) | Euro |
| IMMO-14 | selection of 14 enzyme carriers (for covalent, ionic and adsorbtion binding of enzymes), 25 g each | 699 € |
| IMMO-COV | 11 epoxide carriers for covalent enzyme binding, 25 g each | 549 € |
| IMMO-ADS | 6 adsorbing enzyme carriers, 25 g each | 299 € |
| IMMO-ANI | 11 anionic carriers for ionic binding of enzymes), 25 g each | 549 € |
| IMMO-HIS | 18 nickel loaded carriers for HIS-tag affinity binding of enzymes, 25 g each | 899 € |