Immozymes

Immobilized lipases, proteases and other enzymes

Immobeads

Immobilization and purification of enzymes and proteins in general requires special carriers. ChiralVision offers carriers with high porosity and various binding modes for optimal immobilization and purification.

Our carriers with binding through epoxides, adsorption, cationic groups, anionic groups and His-tags cover the most important range of binding options.

Bead product list (PDF)

Chirals

chiral compounds


 

Immobeads

ChiralVision has an extensive range of enzyme carriers with various surface functionalizations. These are based on acrylic polymers but also on silica and cellulose. In collaboration with Sunresin we also offer their line of life science resins making the range of options for your application very complete.

All carriers are available separately and in convenient screening kits (see bottom page).

Immobeads for covalent, adsorption and ionic immobilization

Macroporous carriers for enzyme immobilization or protein purification. Available in various screening kit combination or separate in bulk.

Covalent  
  All Immobeads are available in bulk
Product# (Sunresin#) Bead description
IB-COV-1 Covalent, very hydrophobic, polyacrylic, low epoxide and high butyl
IB-COV-2 Covalent, hydrophilic, polyacrylic, epoxide and low butyl
IB-COV-3 discontinued, replaced by IB-COV-8
IB-COV-4 Covalent, very hydrophobic, polyacrylic, low epoxide and octadecyl
IB-COV-5 Covalent, hydrophobic, polyacrylic, epoxide and hexadecyl/octadecyl
IB-COV-6 Covalent, very hydrophilic, polyacrylic, epoxide and hydroxyethyl
IB-COV-7  (EMC7014M) Covalent, hydrophilic, polyacrylic, epoxide
IB-COV-8  (EMC7025) Covalent, very hydrophilic, polyacrylic, epoxide 
IB-COV-9  (EMC7032) Covalent, extremely hydrophilic, silica, propyl epoxide
IB-COV-10 Covalent, extremely hydrophilic, cellulose, epoxide, anionic
IB-COV-11 Covalent, hydrophilic, polyacrylic, epoxide
IB-COV-12 Covalent, hydrophobic, polyacrylic, epoxide, butyl

 

Adsorption  
  All Immobeads are available in bulk
Product# (Sunresin#) Bead description
IB-ADS-1 Adsorption, hydrophobic, polyacrylic, hexadecyl/octadecyl
IB-ADS-4 discontinued, replaced by IB-ADS-10
IB-ADS-8 Adsorption, hydrophobic, polyacrylic, low butyl
IB-ADS-9 Adsorption, extremely hydrophobic, polyacrylic, high butyl
IB-ADS-10  (EMC1040) Adsorption, hydrophilic, polystyrene, styrene, methyl
IB-ADS-11  (EMC7528) Adsorption, hydrophobic, polyacrylic, octadecyl
IB-ADS-12  (EMC1020) Adsorption, hydrophobic, polystyrene / DVB, styrene

 

Cationic  
  All Immobeads are available in bulk
Product nr. Bead description
IB-CAT-1 Cationic, strong, polystyrene, sulphonic

 

Anionic  
  All Immobeads are available in bulk
Product# (Sunresin#) Bead description
IB-ANI-5  (EMC7120M) Anionic, hydrophobic, polyacrylic, primary, secondary amine 
IB-ANI-6  (EMC7424) Anionic, hydrophilic, polystyrene / DVB, tertiary amine
IB-ANI-7 Anionic, very hydrophilic, polyacrylic, primary, secondary amine 
IB-ANI-8 Anionic, very hydrophilic, polyacrylic, primary amine
IB-ANI-9 Anionic, hydrophobic, polyacrylic, secondary, tertiary amine
IB-ANI-10 Anionic, hydrophilic, polyacrylic, primary, secondary amine 
IB-ANI-11 Anionic, hydrophobic, polyacrylic, primary, secondary amine 
IB-ANI-12 Anionic, extremely hydrophilic, silica, amino propyl
IB-ANI-13 Anionic, extremely hydrophilic, cellulose, primary, secondary amine 
IB-ANI-14  (EMC7435) Anionic, hydrophobic, polystyrene / DVB, quaternary amine
IB-ANI-15  (EMC7225M) Anionic, hydrophilic, polyacrylic, short diamine spacer

 

Purolite has discontinued various lifescience products. ChiralVision offers the following drop-in replacement beads:

ChiralVision product # Replaces Purolite product #
IB-COV-8 ECR8215
IB-ADS-10 ECR1030
IB-ADS-11 ECR8806
IB-ADS-12 AP1090
IB-HIS-8 Chromalite MIDA Ni

Contact us for remaining Purolite stock!

Immobead product list (PDF)

Immobeads for His-tag affinity purification and immobilization

The Immobead His-tag product line is suited for binding enzymes and proteins with a His-tag domain. The carriers have nickel attached via an iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA) group and are ready for use. If you prefer to obtain these carriers without a metal ion or with a different metal please contact us. The products are available as screening kit or individual in various quantities as well as in bulk.

   
Product# (Sunresin#) Bead description
IB-HIS-1 HIS-tag, very hydrophobic, polyacrylic, low IDA-nickel, high butyl
IB-HIS-2 HIS-tag, hydrophilic, polyacrylic, IDA-nickel, low butyl
IB-HIS-3 HIS-tag, very hydrophobic, polyacrylic, low IDA-nickel, octadecyl
IB-HIS-4 HIS-tag, hydrophobic, polyacrylic, IDA-nickel, hexadecyl/octadecyl
IB-HIS-7 HIS-tag, very hydrophilic, polyacrylic, IDA-nickel, hydroxyethyl
IB-HIS-8 HIS-tag, very hydrophilic, polyacrylic, IDA-nickel
IB-HIS-9 HIS-tag, very hydrophilic, polyacrylic, IDA-nickel, ammonia quenched
IB-HIS-11 HIS-tag, hydrophilic, anionic, polyacrylic, diamine, NTA-nickel
IB-HIS-14 HIS-tag, hydrophilic, anionic, polyacrylic, NTA-nickel
IB-HIS-15 HIS-tag, extremely hydrophilic, anionic, silica, aminopropyl, NTA-nickel
IB-HIS-16 HIS-tag, extremely hydrophilic, anionic, cellulose, NTA-nickel
IB-HIS-17  (Chelex7350) HIS-tag, hydrophilic, anionic, polyacrylic, IDA and Nickel
IB-HIS-18 HIS-tag, hydrophobic, anionic, polyacrylic, diamine, NTA-nickel
IB-HIS-19 HIS-tag, very hydrophilic, anionic, polyacrylic, diamine, NTA-nickel
IB-HIS-20 HIS-tag, very hydrophilic, polyacrylic, ammonia, NTA-nickel
IB-HIS-21 HIS-tag, extremely hydrophilic, silica, propyl, IDA-nickel
IB-HIS-22 HIS-tag, extremely hydrophilic, cellulose, IDA-nickel
IB-HIS-24 HIS-tag, very hydrophilic, polyacrylic, diamine, EDTA-nickel

 

 

His-tag Immobead product list (PDF)

Immobeads: screening kits

Various screening kits are available with a variation in binding types:

 

Catalog No. Immobead enzyme carrier Kit - Pricing in euro (ex. shipping) Euro
IMMO-14 selection of 14 enzyme carriers (for covalent, ionic and adsorbtion binding of enzymes), 25 g each 699 €
IMMO-COV 11 epoxide carriers for covalent enzyme binding, 25 g each 549 €
IMMO-ADS 6 adsorbing enzyme carriers, 25 g each 299 €
IMMO-ANI 11 anionic carriers for ionic binding of enzymes), 25 g each 549 €
IMMO-HIS 18 nickel loaded carriers for HIS-tag affinity binding of enzymes, 25 g each 899 €