Immozymes

For each immobilized enzyme optimization of activity and stability is performed. After immobilization all Immozymes are washed extensively. Finally, they are dried yielding an enzyme that can be stored for prolonged times and used in water-free media.

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Immobeads

enzyme carriers

Chirals

chiral compounds


Discover immobilized lipases

ChiralVision’s immobilized lipases are ideal for applications in aqueous, biphasic and water-free conditions.

All Immozymes are available separate in bulk or in a convenient screening kit. The kit contains 19 covalently immobilized enzymes (on IB-COV-1):
-19 immobilized lipases
-1 gram each
-covalently bound on polyacrylic bead IB-COV-1 (particle size 150 - 600 μm)
-instructions
 

HYDROLYSIS

TBU activity: hydrolysis of tributyrin (“TB” Units) with water to butyric acid and diglycerides.
1 TBU unit = 1 μmol butyric acid released per minute / g immobilized enzyme.
Conditions: 1-10% tributyrin in buffer at 40°C and pH 7.5. Reaction time: 5-30 minutes


ESTERIFICATION

PLU activity: esterification of lauric acid with 1-propanol to propyllaurate (“PL” Units).
1 PLU unit = 1 μmol propyllaurate formed per minute / g immobilized enzyme.
Conditions: 1 eq lauric acid, 1.2 eq 1-propanol and 0.44 eq water at 60°C. Reaction time: 5 minutes

 

Indicative activities of immobilized lipases in:
hydrolysis (TBU) and esterification (PLU).

 

Product nr. Lipases - Immobilized formulations
IMMCALA-COV-1 lipase A from Candida antarctica (CaLA, NovoCor AD L 1), covalent on IB-COV-1. Activity: 2500 TBU / g
IMMCALB-ADS-11 lipase B from Candida antarctica (CaLB1), adsorbed on acrylic beads. Activity: 11000 PLU / g 
IMMCALB-COV-1 lipase B from Candida antarctica (CaLB1), covalent on IB-COV-1. Activity: 2500 TBU / g, 5000 PLU / g
IMMCALB-COV-1XL lipase B from Candida antarctica (CaLB1), covalent on IB-COV-1. Activity: 15000 PLU / g
IMMCALBY-ADS-11 generic lipase B from Candida antarctica (CaLB2), adsorbed on acrylic beads. Activity: 10000 PLU / g
IMMCALBY-COV-1 generic lipase B from Candida antarctica (CaLB2), covalent on IB-COV-1. Activity: 3000 TBU/g, 8000 PLU/g
IMMRML-COV-1 lipase from Rhizomucor miehei (Lipozyme1), covalent on IB-COV-1. Activity: 500 TBU / g
IMMTLL-COV-1 Thermomyces lanuginosa lipase  (Lipolase1), covalent on IB-COV-1. Activity: >5000 TBU/g, 3500 PLU/g
IMMCRL-COV-1 lipase from Candida rugosa (CRL3), covalent on IB-COV-1. Activity: 250 TBU / g
IMMCCMO-COV-1 lipase from C. cylindracea (equal to C. rugosa) (lipase OF6), covalent on IB-COV-1. Activity: 500 TBU / g
IMMABC-COV-1 lipase from Pseudomonas cepacia (Amano PS4), covalent on IB-COV-1. Activity: 1500 TBU / g
IMMAPF-COV-1 lipase from Pseudomonas fluorescens (Amano AK4), covalent on IB-COV-1. Activity: 750 TBU / g
IMMARO-COV-1 lipase from Rhizopus oryzae (Amano F-AP154), covalent on IB-COV-1. Activity: 500 TBU / g
IMMAMJ-COV-1 lipase from Mucor javanicus (Amano M4), covalent on IB-COV-1. Activity: 300 TBU / g
IMMANA-COV-1 lipase from Aspergillus niger (Lipase A4), covalent on IB-COV-1. Activity: 40 TBU / g
IMMRNA-COV-1 lipase from Rhizopus niveus (Lipase4), covalent on IB-COV-1. Activity: 10 TBU / g
IMMASMQ-COV-1 lipase from Alcaligenes sp. (lipase QLM6), covalent on IB-COV-1. Activity: 200 TBU / g
IMMRES-COV-1 lipase Resinase HT (Resinase HT1), covalent on IB-COV-1. Activity: 3000 TBU / g, 6000 PLU / g
IMMLIPX-COV-1 lipase Lipex 100L (Lipex 100L1), covalent on IB-COV-1. Activity: 3000 TBU / g, 6000 PLU / g
IMMEVT-COV-1 lipase Eversa Transform1 (TLL mutant), covalent on IB-COV-1. Activity: 4000 TBU / g, 8000 PLU / g
IMML51-COV-1 lipase from H. insolens (Cutinase, NZ51032, StickAway)1, covalent on IB-COV-1. Activity: 5000 TBU / g, 5000 PLU / g
IMML51-ADS-11 lipase from Humicola insolens (Cutinase, NZ51032, StickAway)1 adsorbed on acrylic beads. Activity: 3000 PLU / g
IMMAULI-COV-1 lipase from Bacillus subtilis (lipase7), covalent on IB-COV-1. Activity: 1000 TBU / g
IMMAULI-ADS-11 lipase from Bacillus subtilis (lipase7) adsorbed on  acrylic beads. Activity: 2000 TBU / g
IMMLIP-1900 Immozyme lipase Kit, 19 immobilized lipases on IB-COV-1. 1 g each
  1) Novozymes; 2) generic 3) Sigma-Aldrich 4) Amano; 5) Fluka; 6) Meito 7) Aum Enzymes
Unit definition: 1 TBU unit = 1 μmol butyric acid released per minute / g immobilized enzyme at 40°C and pH 7.5. 
Unit definition: 1 PLU unit = 1 μmol propyl laurate formed per minute / g immobilized enzyme at 60°C and pH 7.5.

 

 

  Lipase Non-Immobilized 
   
   
Product nr. Lipase (not immobilized)
CV-CALA lipase A from Candida antarctica (CaLA, similar to NovoCor AD L), liquid, >6000 TBU / ml
Unit definition: 1 TBU unit = 1 μmol butyric acid released per minute / ml immobilized enzyme at 40°C and pH 7.5.