Immozymes
Immobilized lipases, proteases and other enzymes
Immobeads
Immobilization and purification of enzymes and proteins in general requires special carriers. ChiralVision offers carriers with high porosity and various binding modes for optimal immobilization and purification.
Our carriers with binding through epoxides, adsorption, cationic groups, anionic groups and His-tags cover the most important range of binding options.
Chirals
chiral compounds
Immobeads overview: polymer properties and functional group tree
ChiralVision has an extensive range of carriers with various surface functionalizations. Whereas some products are directly ready for use after polymerization many other products are derived from these parent beads through modification of the functional groups. Therefor many beads have related properties which are displayed in the table below.
| Surface | Matrix | Product name | Functional group | % water | Particle size (μm) | Derivatization | Product name | Derivatization | Product name |
| Very hydrophobic | Polyacrylic | IB-COV-4 | low epoxide, high octadecyl (OMA) | 75 | 150-600 | IB-COV-4-IDA | IB-HIS-3 | ||
| Very hydrophobic | Polyacrylic | IB-COV-1 | low epoxide, high butyl | 75 | 150-600 | IB-COV-1-IDA | IB-HIS-1 | ||
| Very hydrophobic | Polyacrylic | IB-ADS-9 | high butyl | 60 | 200-355 | ||||
| Hydrophobic | Polyacrylic | IB-COV-5 | 10% epoxide and 10% hexadecyl/octadecyl (CSMA) | 70 | 200-710 | IB-COV-5-IDA | IB-HIS-4 | ||
| IB-COV-5-HMDA | IB-ANI-11 | IB-COV-5-HMDA-NTA/ | IB-HIS-18 | ||||||
| IB-ANI-11-NTA | |||||||||
| IB-COV-5-DMAPA | IB-ANI-9 | ||||||||
| Hydrophobic | Polyacrylic | IB-ADS-1 | low hexadecyl/octadecyl (CSMA) | 65 | 350-700 | ||||
| Hydrophilic | Polyacrylic | IB-COV-2 | high epoxide, low butyl | 75 | 150-710 | IB-COV-2-IDA | IB-HIS-2 | ||
| IB-COV-2-HMDA | IB-ANI-10 | IB-COV-2-HMDA-NTA/ | IB-HIS-11 | ||||||
| IB-ANI-10-NTA | |||||||||
| Hydrophilic | Polyacrylic | IB-ADS-8 | low butyl | 77 | 200-400 | ||||
| Hydrophilic | Polyacrylic | IB-COV-7 | high epoxide | 55-65 | 150-300 | IB-COV-7-IDA | IB-HIS-17 | ||
| IB-COV-7-HMDA | IB-ANI-5 | IB-COV-7-HMDA-NTA/ | IB-HIS-14 | ||||||
| IB-ANI-5-NTA | |||||||||
| IB-COV-7-HMDA-EDTA/ | IB-HIS-23 | ||||||||
| IB-ANI-5-EDTA | |||||||||
| Very hydrophilic | Polyacrylic | IB-COV-8 | high epoxide | 70 | 200-710 | IB-COV-8-IDA | IB-HIS-8 | IB-COV-8-IDA, NH3 quench/ | IB-HIS-9 |
| IB-HIS-8, NH3 quench | |||||||||
| IB-COV-8-HMDA | IB-ANI-7 | IB-COV-8-HMDA-NTA/ | IB-HIS-19 | ||||||
| IB-ANI-7-NTA | |||||||||
| Very hydrophilic | Polyacrylic | IB-COV-6 | medium epoxide, hydroxyethyl | 70 | 200-710 | IB-COV-6-IDA | IB-HIS-7 | ||
| IB-COV-6-NH3 | IB-ANI-8 | IB-COV-6-NH3-NTA/ | IB-HIS-20 | ||||||
| IB-ANI-8-NTA | |||||||||
| Extremely hydrophilic | Silica | IB-SLC-GPTMS/ | Epoxide | 50 | 250-500 | IB-SLC-GPTMS-IDA/ | IB-HIS-21 | ||
| IB-COV-9 | IB-COV-9-IDA | ||||||||
| IB-SLC-APTES/ | Propyl amine | 50 | 250-500 | IB-SLC-APTES-NTA/ | IB-HIS-15 | ||||
| IB-ANI-12 | IB-ANI-12-NTA | ||||||||
| Extremely hydrophilic | Cellulose | IB-CEL-EpiCl | Short epoxide | 50 | 100 | IB-CEL-EpiCl-IDA | IB-HIS-22 | ||
| IB-CEL-EpiCl-HMDA/ | IB-ANI-13 | IB-CEL-EpiCl-HMDA-NTA/ | IB-HIS-16 | ||||||
| IB-ANI-13-NTA | |||||||||
| IB-CEL-EpiCl-HMDA-EpiCl/ | IB-COV-10 | ||||||||
| IB-ANI-13-EpiCl | |||||||||
Immobeads for covalent, adsorption and ionic immobilization
Macroporous carriers for enzyme immobilization or protein purification. Available in various screening kit combination or separate in bulk.
| Product name | Type | Hydrophobicity | Matrix | Functional groups | Water % | Particle size (µm) |
| IB-COV-1 | Covalent | very hydrophobic | polyacrylic | low epoxide and high butyl | 75 | 150-600 |
| IB-COV-2 | Covalent | hydrophilic | polyacrylic | epoxide and low butyl | 75 | 150-710 |
| IB-COV-4 | Covalent | very hydrophobic | polyacrylic | low epoxide and octadecyl | 75 | 150-600 |
| IB-COV-5 | Covalent | hydrophobic | polyacrylic | epoxide and hexadecyl/octadecyl | 70 | 200-710 |
| IB-COV-6 | Covalent | very hydrophilic | polyacrylic | epoxide and hydroxyethyl | 70 | 200-710 |
| IB-COV-7 | Covalent | hydrophilic | polyacrylic | epoxide | 60 | 300-600 |
| IB-COV-8 | Covalent | very hydrophilic | polyacrylic | epoxide | 70 | 200-710 |
| IB-COV-9 | Covalent | extremely hydrophilic | silica | propyl epoxide | 60 | 250-400 |
| IB-COV-10 | Covalent | extremely hydrophilic | cellulose | epoxide, anionic | 50 | 20-40 |
| IB-COV-11 | Covalent | hydrophilic | polyacrylic | epoxide | 66 | 150-250 |
| IB-COV-12 | Covalent | hydrophobic | polyacrylic | epoxide, butyl | 63 | 100-400 |
| IB-ADS-1 | Adsorption | hydrophobic | polyacrylic | hexadecyl/octadecyl | 65 | 350-700 |
| IB-ADS-4 | Adsorption | hydrophilic | polystyrene | styrene, methyl | 58 | 300-700 |
| IB-ADS-8 | Adsorption | hydrophobic | polyacrylic | low butyl | 77 | 200-400 |
| IB-ADS-9 | Adsorption | extremely hydrophobic | polyacrylic | high butyl | 60 | 200-355 |
| IB-ADS-10 | Adsorption | hydrophilic | polystyrene | styrene, methyl | 62 | 300-700 |
| IB-ADS-11 | Adsorption | hydrophobic | polyacrylic | octadecyl | 62 | 300-1000 |
| IB-ADS-12 | Adsorption | hydrophobic | polystyrene / DVB | styrene | 50 | 300-900 |
| IB-ANI-2 | Anionic | weak | polystyrene | tertiary amine | 55 | 400-1000 |
| IB-ANI-3 | Anionic | weak | polystyrene | quaternary amine | 72 | 400-1000 |
| IB-ANI-4 | Anionic | strong | polystyrene | quaternary amine | 62 | 300-1200 |
| IB-ANI-5 | Anionic | hydrophobic | polyacrylic | primary, secondary amine | 60 | 300-600 |
| IB-ANI-6 | Anionic | hydrophilic | polystyrene / DVB | tertiary amine | 50 | 500-1000 |
| IB-ANI-7 | Anionic | very hydrophilic | polyacrylic | primary, secondary amine | 70 | 200-710 |
| IB-ANI-8 | Anionic | very hydrophilic | polyacrylic | primary amine | 70 | 200-710 |
| IB-ANI-9 | Anionic | hydrophobic | polyacrylic | secondary, tertiary amine | 70 | 200-710 |
| IB-ANI-10 | Anionic | hydrophilic | polyacrylic | primary, secondary amine | 75 | 150-710 |
| IB-ANI-11 | Anionic | hydrophobic | polyacrylic | primary, secondary amine | 70 | 200-710 |
| IB-ANI-12 | Anionic | extremely hydrophilic | silica | amino propyl | 60 | 250-400 |
| IB-ANI-13 | Anionic | extremely hydrophilic | cellulose | primary, secondary amine | 50 | 20-40 |
| IB-ANI-14 | Anionic | hydrophobic | polystyrene / DVB | quaternary amine | 70 | 315-1250 |
| IB-CAT-1 | Cationic | strong | polystyrene | sulphonic | 56 | 300-700 |
Purolite has discontinued various lifescience products. ChiralVision offers the following drop-in replacement beads:
| ChiralVision product # | Replaces Purolite product # |
| IB-COV-8 | ECR8215 |
| IB-ADS-10 | ECR1030 |
| IB-ADS-11 | ECR8806 |
| IB-ADS-12 | AP1090 |
| IB-HIS-8 | Chromalite MIDA Ni |
Contact us for remaining Purolite stock!
Immobeads for His-tag affinity purification and immobilization
The Immobead His-tag product line is suited for binding enzymes and proteins with a His-tag domain. The carriers have nickel attached via an iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA) group and are ready for use. If you prefer to obtain these carriers without a metal ion or with a different metal please contact us. The products are available as screening kit or individual in various quantities as well as in bulk.
| Product name | Type | Hydrophobicity | Matrix | Functional groups | Water % | Particle size (µm) |
|
IB-HIS-1 |
HIS-tag |
very hydrophobic |
polyacrylic |
low IDA-nickel, high butyl |
75 |
150-600 |
|
IB-HIS-2 |
HIS-tag |
hydrophilic |
polyacrylic |
IDA-nickel, low butyl |
75 |
150-710 |
|
IB-HIS-3 |
HIS-tag |
very hydrophobic |
polyacrylic |
low IDA-nickel, octadecyl |
75 |
150-600 |
|
IB-HIS-4 |
HIS-tag |
hydrophobic |
polyacrylic |
IDA-nickel, hexadecyl/octadecyl |
70 |
200-710 |
|
IB-HIS-7 |
HIS-tag |
very hydrophilic |
polyacrylic |
IDA-nickel, hydroxyethyl |
70 |
200-710 |
|
IB-HIS-8 |
HIS-tag |
very hydrophilic |
polyacrylic |
IDA-nickel |
70 |
200-710 |
|
IB-HIS-9 |
HIS-tag |
very hydrophilic |
polyacrylic |
IDA-nickel, ammonia quenched |
70 |
200-710 |
|
IB-HIS-11 |
HIS-tag |
hydrophilic, anionic |
polyacrylic |
diamine, NTA-nickel |
75 |
150-710 |
|
IB-HIS-14 |
HIS-tag |
hydrophilic, anionic |
polyacrylic |
NTA-nickel |
60 |
300-600 |
|
IB-HIS-15 |
HIS-tag |
extremely hydrophilic, anionic |
silica |
aminopropyl, NTA-nickel |
60 |
250-400 |
|
IB-HIS-16 |
HIS-tag |
extremely hydrophilic, anionic |
cellulose |
NTA-nickel |
50 |
20-40 |
|
IB-HIS-17 |
HIS-tag |
hydrophilic, anionic |
polyacrylic |
IDA and Nickel |
60 |
100-250 |
|
IB-HIS-18 |
HIS-tag |
hydrophobic, anionic |
polyacrylic |
diamine, NTA-nickel |
70 |
200-710 |
|
IB-HIS-19 |
HIS-tag |
very hydrophilic, anionic |
polyacrylic |
diamine, NTA-nickel |
70 |
200-710 |
|
IB-HIS-20 |
HIS-tag |
very hydrophilic |
polyacrylic |
ammonia, NTA-nickel |
70 |
200-710 |
|
IB-HIS-21 |
HIS-tag |
extremely hydrophilic |
silica |
propyl, IDA-nickel |
55 |
250-400 |
|
IB-HIS-22 |
HIS-tag |
extremely hydrophilic |
cellulose |
IDA-nickel |
50 |
20-40 |
|
IB-HIS-24 |
HIS-tag |
very hydrophilic |
polyacrylic |
diamine, EDTA-nickel |
70 |
200-710 |
His-tag Immobead product list (PDF)
Immobeads: screening kits
Various screening kits are available with a variation in binding types:
| Catalog No. | Immobead enzyme carrier Kit - Pricing in euro (ex. shipping) | Euro |
| IMMO-14 | selection of 14 enzyme carriers (for covalent, ionic and adsorbtion binding of enzymes), 25 g each | 699 € |
| IMMO-COV | 11 epoxide carriers for covalent enzyme binding, 25 g each | 549 € |
| IMMO-ADS | 7 adsorbing enzyme carriers, 25 g each | 349 € |
| IMMO-ANI | 13 anionic carriers for ionic binding of enzymes), 25 g each | 649 € |
| IMMO-HIS | 18 nickel loaded carriers for HIS-tag affinity binding of enzymes, 25 g each | 899 € |